splenocyte suspensions Search Results


single-cell suspensions of splenocytes  (Orient Bio Company)
90
Orient Bio Company single-cell suspensions of splenocytes
Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from <t>splenocytes</t> of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Single Cell Suspensions Of Splenocytes, supplied by Orient Bio Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-cell suspensions of splenocytes/product/Orient Bio Company
Average 90 stars, based on 1 article reviews
single-cell suspensions of splenocytes - by Bioz Stars, 2026-03
90/100 stars
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splenocyte suspensions  (Harlan UK Ltd)
90
Harlan UK Ltd splenocyte suspensions
Inhibition of <t> splenocyte </t> proliferation induced by concanavalin A (Con A) and lipopolysaccharide (LPS) in the presence of crude tick salivary glands extracts (SGE) or B-cell inhibitory protein (BIP)-enriched material
Splenocyte Suspensions, supplied by Harlan UK Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte suspensions/product/Harlan UK Ltd
Average 90 stars, based on 1 article reviews
splenocyte suspensions - by Bioz Stars, 2026-03
90/100 stars
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splenocyte cell suspensions  (Charles River Laboratories)
90
Charles River Laboratories splenocyte cell suspensions
Inhibition of <t> splenocyte </t> proliferation induced by concanavalin A (Con A) and lipopolysaccharide (LPS) in the presence of crude tick salivary glands extracts (SGE) or B-cell inhibitory protein (BIP)-enriched material
Splenocyte Cell Suspensions, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte cell suspensions/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
splenocyte cell suspensions - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
splenocyte suspension  (ImmunoGen Inc)
90
ImmunoGen Inc splenocyte suspension
Inhibition of <t> splenocyte </t> proliferation induced by concanavalin A (Con A) and lipopolysaccharide (LPS) in the presence of crude tick salivary glands extracts (SGE) or B-cell inhibitory protein (BIP)-enriched material
Splenocyte Suspension, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte suspension/product/ImmunoGen Inc
Average 90 stars, based on 1 article reviews
splenocyte suspension - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from splenocytes of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Journal of Microbiology and Biotechnology

Article Title: Deinococcus radiodurans R1 Lysate Induces Tolerogenic Maturation in Lipopolysaccharide-Stimulated Dendritic Cells and Protects Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.4014/jmb.2203.03008

Figure Lengend Snippet: Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from splenocytes of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Following the established methodology, single-cell suspensions of splenocytes were obtained from 7-week-old BALB/c mice (Orient Bio, Inc.) [ ].

Techniques: Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence, Isolation, Staining, Comparison

Inhibition of splenocyte proliferation induced by concanavalin A (Con A) and lipopolysaccharide (LPS) in the presence of crude tick salivary glands extracts (SGE) or B-cell inhibitory protein (BIP)-enriched material

Journal:

Article Title: Characterization of the B-cell inhibitory protein factor in Ixodes ricinus tick saliva: a potential role in enhanced Borrelia burgdoferi transmission

doi: 10.1111/j.1365-2567.2004.01975.x

Figure Lengend Snippet: Inhibition of splenocyte proliferation induced by concanavalin A (Con A) and lipopolysaccharide (LPS) in the presence of crude tick salivary glands extracts (SGE) or B-cell inhibitory protein (BIP)-enriched material

Article Snippet: B cells were isolated from splenocyte suspensions from 8 to 15-week-old female BALB/c mice (Harlan UK, Bicester, UK) on a 70-μm nylon cell strainer in cold RPMI-1640 (Life Technologies).

Techniques: Inhibition